Abstract : 240 healthy weaned pigs with similar body weight (11.64 ± 0.37 kg) were selected for this study. Using a single factor completely randomized design, it was divided into antibiotic group, test group, test group 2, and test group with 4 treatment groups (male and female half). Each group of peptides (trade name, the main component is cecropin antibacterial peptide) was added in different amounts. The test period is 28 days. The results showed that the feed conversion ratio of the first, second and third groups was 18.29%, 7.93% and 14.02% lower than the antibiotic control group, and the effect of the group was the best (P < 0.01). Therefore, it can be judged by this experiment that the addition of 250 mg/kg of peptide preparation to the piglet diet can significantly improve the feed conversion rate, reduce the feed cost, and not affect the daily gain, which proves that the peptide is increasing the growth of weaned piglets. A unique advantage in performance. sLi China Feed Industry Information Network - based on feed, serving animal husbandry
Key words : cecropin antibacterial peptide weaned piglet growth performance sLi China feed industry information network - based on feed, service animal husbandry
Cecropin antibacterial peptide is a kind of peptide substance produced by the biological defense system against foreign pathogens. It has many activities against bacteria, fungi, molds, viruses, protozoa, cancer cells, etc., and is not easy to produce drug resistance. Application prospects [1]. Li Guodong et al [2], after testing, proved that some of the sequences containing the core standard spiral structure, the minimum inhibitory concentration against Gram-positive and negative bacteria is only about 1/100 of the original cecropin A1 antimicrobial peptide. There is hope for further development as a new anti-infective drug. Wan Ruru et al [3], in the veterinary clinical practice, proved that antimicrobial peptides have a good control effect on animal diseases. And in the prevention and treatment of pig disease has played a good effect. The cecropin antibacterial peptide was first discovered by the Swedish scientist Boman [5], which was found in 1980 after the use of C. vaginalis and Escherichia coli to induce the sputum. The study found that it not only has a spectral antibacterial effect, but also a virus, fungus, Tumors and the like all have a certain killing effect. Cecropin antibacterial peptide is also one of the most thoroughly studied and most effective antibacterial peptides. sLi China Feed Industry Information Network - based on feed, serving animal husbandry
After careful research by experts and scholars, the peptides (the main component of cecropin antibacterial peptide) are artificially designed and synthesized and successfully expressed in Bacillus subtilis [4]. In vitro tests have proven to be effective in inhibiting harmful bacteria such as Escherichia coli, Staphylococcus aureus, and Salmonella. Moreover, the peptide preparation has no toxic and side effects, and there is no harmful residue in the livestock and poultry, which is in line with the development direction of green foods and meets the needs of domestic and foreign markets, and is an ideal way to develop green feed additives. Therefore, it is an excellent choice for antibiotic replacement. sLi China Feed Industry Information Network - based on feed, serving animal husbandry
2 Materials and methods sLi China feed industry information network - based on feed, service animal husbandry
2.1 Test animals and materials sLi China feed industry information network - based on feed, service animal husbandry
A total of 240 healthy weaned piglets of Dux × Landrace × Yorkshire with an initial body weight of 11.64 ± 2.30 kg were used for the test. sLi China Feed Industry Information Network - based on feed, serving animal husbandry
2.2 Test site and test time sLi China Feed Industry Information Network - based on feed, service animal husbandry
Test site: a large-scale one-stop enterprise in Changsha, Hunan Province sLi China Feed Industry Information Network - based on feed, serving animal husbandry
Test time: May 20, 2010 to June 17, 2010 sLi China Feed Industry Information Network - based on feed, serving animal husbandry
2.3 experimental design and diet sLi China feed industry information network - based on feed, service animal husbandry
The pigs selected in the experiment were randomly divided into 4 treatments according to the principle of similar body weight and similar genetic basis. Each treatment was repeated 6 times, and 10 pigs per pig (male and female) were used. The experimental design was: control group. The antibiotic group (basic diet + 200 mg / kg 10% sulfuric acid antibiotics + 1000 mg / kg 10% bacitracin zinc); test a group (basic diet + 250 mg / kg of peptides); Test group 2 (basic diet + 500 mg/kg of peptides); test three groups (basal diet + 750 mg/kg of peptides). Managed and recorded by special personnel, other daily management is carried out as normal. The trial used a corn-soybean-based basal diet to prepare a porcine trial basal diet and premix with reference to the NRC (1998) porcine nutritional requirements. The experimental basic diet formula and nutritional level are shown in Table 1. The 4% premix formulation of each test group diet is shown in Table 2. The peptide biotin was provided by Beijing Zhongnong Yingtai Biotechnology Co., Ltd. sLi China Feed Industry Information Network - based on feed, serving animal husbandry
Table 1 Basic diet formula and nutritional level sLi China Feed Industry Information Network - based on feed, service animal husbandry
Raw materials (%) sLi China Feed Industry Information Network - based on feed, serving livestock | Content sLi China Feed Industry Information Network - based on feed, serving animal husbandry | Nutrition level sLi China feed industry information network - based on feed, service animal husbandry | Content sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Corn sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 33.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | CP,% sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 19.71 sLi China Feed Industry Information Network - Based on feed, serving livestock |
Puffed corn sLi China feed industry information network - based on feed, serving animal husbandry | 26 sLi China Feed Industry Information Network - based on feed, serving livestock | DE, Mcal /kg sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 3.39 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry |
Peeled soybean meal sLi China feed industry information network - based on feed, service animal husbandry | 12 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | Lys, % sLi China Feed Industry Information Network - based on feed, serving livestock | 1.37 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry |
Puffed whole fat soybean sLi China feed industry information network - based on feed, serving animal husbandry | 12 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | Met, % sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0.43 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Whey powder sLi China feed industry information network - based on feed, service animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | Thr, % sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0.91 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Soy protein concentrate sLi China feed industry information network - based on feed, service animal husbandry | 2.5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | Trp, % sLi China Feed Industry Information Network - based on feed, serving livestock | 0.25 sLi China Feed Industry Information Network - Based on feed, serving livestock |
Fishmeal sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | Ca, % sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0.8 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
4% Premix sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 4 sLi China Feed Industry Information Network - based on feed, serving livestock | P, % sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0.6 sLi China Feed Industry Information Network - based on feed, serving livestock |
Total sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 100 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | sLi China Feed Industry Information Network - based on feed, serving animal husbandry | sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
sLi China Feed Industry Information Network - based on feed, serving animal husbandry
sLi China Feed Industry Information Network - based on feed, serving animal husbandry
Table 2, 4% premix formula sLi China feed industry information network - based on feed, service animal husbandry
Piglet 4% premix sLi China Feed Industry Information Network - based on feed, service animal husbandry | Control group sLi China feed industry information network - based on feed, service animal husbandry (g/kg) sLi China Feed Industry Information Network - based on feed, serving animal husbandry | Test a group of sLi China feed industry information network - based on feed, service animal husbandry (g/kg) sLi China Feed Industry Information Network - based on feed, serving animal husbandry | Test two groups sLi China feed industry information network - based on feed, service animal husbandry (g/kg) sLi China Feed Industry Information Network - based on feed, serving animal husbandry | Test three groups of sLi China feed industry information network - based on feed, service animal husbandry (g/kg) sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Copper sulphate pentahydrate sLi China feed industry information network - based on feed, service animal husbandry | 25 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 25 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 25 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 25 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Zinc sulfate monohydrate sLi China feed industry information network - based on feed, serving animal husbandry | 9.85 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 9.85 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 9.85 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 9.85 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Ferrous sulfate monohydrate sLi China feed industry information network - based on feed, serving animal husbandry | 10.80 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10.80 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10.80 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10.80 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Manganese sulfate monohydrate sLi China feed industry information network - based on feed, serving animal husbandry | 2.6 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 2.6 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 2.6 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 2.6 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry |
Calcium iodate sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Sodium selenite sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
50% choline chloride sLi China feed industry information network - based on feed, service animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
10% sulfuric acid anti- enantiomer sLi China feed industry information network - based on feed, service animal husbandry | 5 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
10% bacitracin zinc sLi China feed industry information network - based on feed, service animal husbandry | 25 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Cecropin sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 0 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 6.25 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 12.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 18.75 sLi China Feed Industry Information Network - Based on feed, serving livestock |
Antioxidant sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 7.5 sLi China Feed Industry Information Network - Based on feed, serving livestock | 7.5 sLi China Feed Industry Information Network - Based on feed, serving livestock | 7.5 sLi China Feed Industry Information Network - Based on feed, serving livestock | 7.5 sLi China Feed Industry Information Network - Based on feed, serving livestock |
Multivitamin sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 6 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 6 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 6 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 6 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Frankincense sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Sweetener sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 10 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Zinc Oxide (first two weeks) sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Calcium hydrogen phosphate 17%p, 21% Ca sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 212.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 212.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 212.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 212.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry |
Stone powder sLi China feed industry information network - based on feed, serving animal husbandry | 207.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 207.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 207.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 207.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry |
Salt sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
L-LYS, 98% sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 50 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 50 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 50 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 50 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
DL-Met, 98% sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 15 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
L-Thr, 98% sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 37.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 37.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 37.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 37.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry |
Maifanshi sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 190.75 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 214.5 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 208.25 sLi China Feed Industry Information Network - Based on feed, serving animal husbandry | 202 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
Total sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 1000 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 1000 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 1000 sLi China Feed Industry Information Network - based on feed, serving animal husbandry | 1000 sLi China Feed Industry Information Network - based on feed, serving animal husbandry |
sLi China Feed Industry Information Network - based on feed, serving animal husbandry
sLi China Feed Industry Information Network - based on feed, serving animal husbandry
sLi China Feed Industry Information Network - based on feed, serving animal husbandry
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